Table 1.
Oligonucleotide primers used for genotyping of Helicobacter pylori isolated from vegetables and salads in Iran.
| Genes names | Primer sequence (5′-3′) | Size of product (bp) |
|---|---|---|
| ureC | F∗: GCTTACTTTCTAACACTAACGCGC R∗∗: GGATAAGCTTTTAGGGGTGTTAGGGG |
296 |
|
| ||
| vacA s1a | F: CTCTCGCTTTAGTAGGAGC R: CTGCTTGAATGCGCCAAAC |
213 |
|
| ||
| vacA s1b | F: AGCGCCATACCGCAAGAG R: CTGCTTGAATGCGCCAAAC |
187 |
|
| ||
| vacA s1c | F: CTCTCGCTTTAGTGGGGYT R: CTGCTTGAATGCGCCAAAC |
213 |
|
| ||
| vacA s2 | F: GCTAACACGCCAAATGATCC R: CTGCTTGAATGCGCCAAAC |
199 |
|
| ||
| vacA m1A | F: GGTCAAAATGCGGTCATGG R: CCATTGGTACCTGTAGAAAC |
290 |
|
| ||
| vacA m1B | F: GGCCCCAATGCAGTCATGGA R: GCTGTTAGTGCCTAAAGAAGCAT |
291 |
|
| ||
| vacA m2 | F: GGAGCCCCAGGAAACATTG R: CATAACTAGCGCCTTGCA |
352 |
|
| ||
| cagA | F: GATAACAGCCAAGCTTTTGAGG R: CTGCAAAAGATTGTTTGGCAGA |
300 |
|
| ||
| iceA1 | F: GTGTTTTTAACCAAAGTATC R: CTATAGCCASTYTCTTTGCA |
247 |
|
| ||
| iceA2 | F: GTTGGGTATATCACAATTTAT R: TTRCCCTATTTTCTAGTAGGT |
229/334 |
|
| ||
| oipA | F: GTTTTTGATGCATGGGATTT R: GTGCATCTCTTATGGCTTT |
401 |
∗F: forward.
∗∗R: reverse.