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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Exp Cell Res. 2011 Jan 7;317(7):1040–1048. doi: 10.1016/j.yexcr.2010.12.026

Figure 1. TNFα induced ET-1 synthesis in stellate cells.

Figure 1

Isolated primary stellate cells were grown for 6 days in medium containing 20% serum to induce activation. Cells were serum starved overnight, then stimulated with TNFα (15 ng/mL) in 0.5% medium for different time points. In (A) preproET-1 mRNA was detected by RT-PCR at the indicated time points as in Methods (n = 3; *p < 0.05 compared to “0). In (B), conditioned supernatant (at 24 hours) was collected and immunoreactive ET-1 was detected as in methods (n = 3; *p < 0.05 compared to control).