Table 1.
High Throughput Sequencing Platforms; Chemistry, Read Lengths and Run times
| Platform | Chemistry | Read length (bp) | Per full run | Run time |
|---|---|---|---|---|
| Roche | Pyrosequencing | |||
| GS Junior System | 400 | 35 Mb | 10 h | |
| GS FLX+ System | Up to 1,000 (with GS FLX Titanium XL+) | 700 Mb | 23 h | |
| Illumina | Sequencing by synthesis | |||
| MiSeq | 2 × 300 (MISEQ REAGENT KIT V3) | 13.2–15 Gb | 65 h | |
| Illumina HiSeq2500 | ||||
| Dual Flow Cell | ||||
| HIGH OUTPUT RUN MODE | 2 × 100 | 540–600 Gb | 11 d | |
| RAPID RUN MODE | 2 × 150 | 150–180 Gb | 40 h | |
| ABI SOLiD | Sequencing by ligation | |||
| 5500xl System | Mate-paired: 2 × 60 bp | 10–15 Gb/day | 2– 7d | |
| Paired-end: 75 bp × 35 bp | ||||
| Fragment: 75 bp | ||||
| Ion Torrent | Semiconductor Sequencing Technology | |||
| Ion PGM | 400 bp (Ion 318™ Chip v2) | 600 Mb- 1 Gb | 7.3 h | |
| Ion Proton | Up to 200-base fragment reads (with Ion PI™ Chip) | Up to 10 Gb | 2–4 h | |
| Pacific Biosciences | Single Molecule Real Time | |||
| Pacific Biosciences PacBio RS | 4200 bp mean mapped read length (with XL Binding/C2 Sequencing Chemistry) | 220 Mb | 2 × 55 m (per SMRT cell) | |
| 7600 bp mean mapped read length (with P5/C3 Sequencing Chemistry) | 608 Mb | 180 m (per SMRT cell) |