Table 3.

Full factorial design for xylanase production by B. pumilus VLK-1 under submerged fermentation

Run	Peptone	Yeast extract	KNO3	Wheat bran	Xylanase activity (IU/ml)
					Observed	Predicted
1	0.725	0.45	0.275	3	2,881.2	3,144.96
2	0.275	0.45	0.275	3	6,667.3	6,667.3
3	0.05	0.8	0.05	1	1,262	1,601.93
4	0.05	0.1	0.05	1	780	799
5	0.05	0.8	0.5	1	3,884	3,990
6	0.275	0.45	−0.175	3	1,576	1,640.39
7	0.5	0.1	0.5	1	956	1,013.28
8	0.275	0.45	0.275	3	6,667.3	6,667.3
9	0.5	0.1	0.5	5	1,988	1,811.44
10	0.275	−0.25	0.275	3	2,560	2,462.06
11	0.5	0.1	0.05	5	4,489.2	4,362.77
12	−0.175	0.45	0.275	3	4,328	3,921.23
13	0.275	0.45	0.275	3	6,667.3	6,667.3
14	0.05	0.8	0.05	5	5,240	5,162.37
15	0.5	0.8	0.5	5	4,048.2	4,008.74
16	0.275	0.45	0.275	3	6,667.3	6,667.3
17	0.05	0.1	0.05	5	1,828.68	1,864.5
18	0.275	0.45	0.275	3	6,667.3	6,667.3
19	0.275	0.45	0.275	3	6,667.3	6,667.3
20	0.275	0.45	0.725	3	1,734.96	1,502.39
21	0.5	0.8	0.5	1	588	715.55
22	0.05	0.1	0.5	5	1,549.44	1,521.79
23	0.5	0.1	0.05	1	1,604	1,704.73
24	0.275	0.45	0.275	7	3,404.4	3,818
25	0.275	0.45	0.275	−1	16	−567.94
26	0.05	0.1	0.5	1	1,818	2,316.27
27	0.5	0.8	0.05	5	6,024	5,689.09
28	0.5	0.8	0.05	1	528.72	536
29	0.275	1.15	0.275	3	5,465.6	5,441.36
30	0.05	0.8	0.5	5	5,628	1,521.79