Table 2.
Effect of cytokinins on shoot multiplication of Stevia rebaudiana
| Induction medium | Multiplication medium | Number of shoots | Shoot length (cm) | Number of leaves |
|---|---|---|---|---|
| BAP (0.2 mg/l) | B | 5.75 ± 0.25bc | 4.32 ± 0.48a | 46.25 ± 0.62cd |
| ½ MS | 3.25 ± 0.62a | 4.20 ± 0.16a | 37.50 ± 1.70b | |
| ½ MS + TDZ (0.01) | 9.25 ± 0.25d | 6.67 ± 0.26d | 50.50 ± 0.95ef | |
| Kn (0.2 mg/l) | E | 4.75 ± 0.25b | 5.05 ± 0.09b | 44.75 ± 0.48c |
| ½ MS | 2.50 ± 0.28a | 4.82 ± 0.16ab | 30.50 ± 0.95a | |
| ½ MS + TDZ (0.01) | 8.50 ± 0.28d | 6.95 ± 0.95d | 50.00 ± 0.81ef | |
| BAP and Kn (0.2 mg/l each) | H | 5.25 ± 0.25bc | 5.20 ± 0.14b | 40.75 ± 0.75b |
| ½ MS | 3.00 ± 0.40a | 5.90 ± 0.12c | 30.50 ± 1.70a | |
| ½ MS + TDZ (0.01) | 8.75 ± 0.25d | 8.05 ± 0.22e | 49.00 ± 1.29de | |
| TDZ (0.5 mg/l) | P | 6.00 ± 0.40c | 4.85 ± 0.12ab | 52.50 ± 1.25f |
| ½ MS | 4.75 ± 0.25b | 5.02 ± 0.16b | 40.00 ± 0.81b | |
| ½ MS + TDZ (0.01) | 11.00 ± 0.40e | 7.17 ± 0.16d | 61.00 ± 1.29g |
Parameters have been recorded after 4 weeks of transfer in multiplication media. Data are in the form of mean ± SEM, and means followed by the same letter within the columns are not significantly different (P = 0.05) using Duncan’s multiple range test