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Stevioside content in in vivo and in vitro leaf samples
| S. no. | Samples | Medium | Retention time (mm:ss) | Peak area (mAs) | Stevioside content (%) |
|---|---|---|---|---|---|
| 1 | Standard | – | 2:22 | 596.1 | – |
| 2 | In vivo plants | – | 2:21 | 8,421.6 | 7.017 ± 0.058 |
| 3 | In vitro plants (3-week-old) | Half strength MS | 2:24 | 11,040.7 | 9.236 ± 0.046 |
| 4 | In vitro-raised plants (8-week-old) | Half strength MS | 2:24 | 11,040.7 | 9.236 ± 0.046 |
Two replicates of each sample were used for HPLC analysis (mean value calculated)
