Table 1.

Details of RAPD and ISSR analysis in 10 species of Zingiberaceae

Markers	Primer	Primer sequence	Approx fragment Size (bp)	Total bands	Unique bands	Resolving power	Primer index
RAPD	OPA4	5′AATCGGGCTG3′	320 to 2,100	29	10	10.72	8.26
	OPA7	5′GAAACGGGTG3′	300 to >3,000	21	12	7.09	5.45
	OPA9	5′GGGTAACGCC3′	600 to 2,100	12	6	4.00	3.07
	OPA18	5′AGGTGACCGT3′	200 to 2,250	25	8	9.81	7.33
	OPC2	5′GTGAGGCGTC3′	500 to >3,000	22	8	10.36	7.07
	OPC5	5′GATGACCGCC3′	900 to 2,500	14	4	5.81	4.36
	OPC11	5′AAAGCTGCGG3′	450 to 2,100	17	3	9.27	6.11
	OPD3	5′GTCGCCGTCA3′	400 to 1,950	13	6	4.90	3.66
	OPD7	5′TTGGCACGGG3′	400 to 2,200	17	2	9.09	6.34
	OPD8	5′GTGTGCCCCA3′	500 to 2,800	18	10	6.90	4.69
	OPD12	5′CACCGTATCC3′	850 to 1,031	3	2	0.72	0.62
	OPD18	5′GAGAGCCAAC3′	450 to >3,000	16	4	6.90	4.86
	OPD20	5′ACCCGGTCAC3′	400 to 3,000	24	6	11.27	7.90
	OPN4	5′GACCGACCCA3′	500 to 2,200	16	3	8.54	5.58
	OPN16	5′AAGCGACCTG3′	200 to 2,100	18	11	6.54	4.72
	OPN18	5′GGTGAGGTCA3′	400 to 1,800	14	3	5.81	4.26
	OPAF5	5′CCCGATCAGA3′	200 > 3,000	15	5	6.90	4.36
	OPAF14	5′GGTGCGCACT3′	400 to 3,000	13	4	4.90	3.80
	OPAF15	5′CACGAACCTC3′	550 to 2,000	10	4	3.45	2.71
	Total		–	317*	111	–	–
	Mean		–	16.7	5.8	6.99	5
	Range		200 to >3,000	3–29	2–12	0.72–11.27	0.62–8.26
ISSR	SPS 1	(GAC)5	200 to 1,200	16**	2	14.72	4.92
	SPS 2	(GTGC)4	250 to 2,000	17	7	10.90	5.52
	SPS 3	(GACA)4	300 to 3,000	22	4	16.54	8.16
	SPS 4	(AGG)6	325 to 1,700	21	6	13.27	7.20
	SPS 5	(GA)9T	350 to 2,000	19	2	12.00	6.64
	SPS 6	T(GA)9	325 to 1,400	17	6	10.54	6.67
	SPS 7	(GTG)5	250 to 1,200	15**	6	11.09	4.79
	SPS 8	(GGA)4	300 to 1,800	20**	3	18.36	7.93
	Total		–	147	36	–	–
	Mean		–	18.37	4.5	13.42	6.47
	Range		200 to >3,000	15–22	2–7	10.54–18.36	4.79–8.16

* All are polymorphic bands, ** contain one monomorphic band, rest are all polymorphic