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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Biochem J. 2014 Mar 15;458(3):575–583. doi: 10.1042/BJ20121546

Figure 5. Biotinylation assay of the surface protein expression for three distinct splice variants of Best1 expressed in HEK293 cells.

Figure 5

A, Western blot analysis of precipitated biotinylated fraction and the crude cell lysates in HEK293 cells expressing the DDK-tagged Best1V1. Representative of four blots. Because of the difference in the expression levels/intensity of the signal, Best1V1-DDK has been probed separately from Best1V1Δex2-DDK and Best1V2. B, Western blot analysis of biotinylated (surface) material and the crude cell lysates in HEK293 expressing Best1V1Δex2-DDK and Best1V2-DDK. Representative of three blots. Asterisks in (B) indicate two very weak immunoreactive bands in cells expressing Best1V2-DDK. Boxes below each panel show actin immunoreactivity on the same membrane after stripping. These controls show lack of cytosolic proteins in biotinylated fraction.