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Kinetic analysis sirohaem decarboxylase. Data from the reaction of 23 μM sirohaem with 2.3 μM D. desulfuricans AhbA/B. Initially, sirohaem (circles) is decarboxylated to monodecarboxysirohaem (triangles), which is in turn decarboxylated to didecarboxysirohaem (squares). These reactions follow a competitive inhibition model for both substrates and the product. Data were fitted using in-house software.
