Variable	Hao 2008	Konig 2010	Shapira 2010	Karlas 2010	Brass 2010
Functional assay(s)	Plate reader, luciferase levels, recombinant VSV-G pseudotyped virus expressing green fluorescence protein and luciferase protein.	Plate reader, Luciferase levels, WSN/Renilla virus	Plate reader, Luciferase levels for viral replication, IFN-β production after ΔNS1 virus infection or viral RNA transfection, PR8 and ΔNS1 viruses.	Imaging of immunostaining for NP levels (part one), Luciferase levels (part two). WSN/33 virus.	Imaging of immunostaining of HA levels, PR8 virus.
Bioinformatic Analyses of screen data	NA	Hynet, Reactome, BIND, MINT, MCODE	BioGRID, BIND, Ingenuity, MSigDB, and INTACT	Gene Ontology, Reactome, String	GOhyperGAll module of Bioconductor for gene ontology UniProt, KEGG, Reactome, Gene Ontology, and NCBI GeneRIF OMIM Human orthologs were mapped to other species using NCBI HomoloGene
siRNA Hit selection	Inhibition exceeding 2.5 standard deviations from the mean in at least One of two replicates	RSA hit criterion of an activity score <0.4	Two fold increase or decrease from the mean for any of the three above noted assays.	Z-scores below -2	Percentage of HA-positive cells was less than 55% or greater than 200% of the plate mean, and cell numbers were not less than 40% of the plate mean.
siRNA transfection Controls	siRNAs against viral NP, and non-targeting negative control	siRNAs against Firefly Renilla and RPS27a, negative controls: scramble 177, scramble5701	NA	siRNAs against viral NP, host PLK1 and the Allstars non-targeting control	siRNAs against viral NP, host NXF1, non-targeting negative control.
Cell types	Drosophila DL1 cells	Human A549 lung carcinoma cells	Human Bronchial Epethelial cells (part one), 293T cells part two.	Human A549 lung carcinoma cells part one, MDCK cells part two.	Human U2OS osteosarcoma cells
siRNA Transfection lipid	None required	0.166% RNAi max (Invitrogen)	Hiperfect (Qiagen), concentration not available	0.109% Hiperfect (Qiagen)	0.32% Oligofectamine (Invitrogen).
siRNA library and siRNA concentration	Ambion Drosophila siRNA library, 200 ng/reaction, 384 well plate	Qiagen Human Genome 1.0 and Human Druggable Genome siRNA set versions 1 and 2, NM Set version 1, XM Set version, a kinome library from Invitrogen and the kinome library from IDT, 1 picomole/reaction, 384 well plate	Dharmacon siARRAY siRNA Library; Human Genome, G-005000-05 candidate set (1745 genes) based on expression and protein interaction experiments, 25 nM	Qiagen Human Genome 1.0 and Human Druggable Genome siRNA Set V2.0, 20 nM, 384 well plate	Dharmacon siARRAY siRNA Library; Human Genome, G- 005000-05, 50 nM. 384 well plate