FIG 5 .

Growth and sdh2-lacZ expression of Δsdh1 mutant. Shown are the growth curves (A) and plotted growth rates (B) of the WT (solid lines and filled bars) and the Δsdh1 deletion mutant (dotted lines and open bars) in HdB minimal medium under normoxic conditions with succinate at 30 mM (closed circles), 15 mM (closed squares), 5 mM (closed triangles), and 1 mM (inverted closed triangles). The specific growth rate was calculated between the two time points indicated in panel A. (C and D) Expression levels of sdh2-lacZ in the M. smegmatis Δsdh1 mutant grown under normoxic (C) or hypoxic (D) conditions in HdB minimal medium supplemented with 30 mM succinate. (D) Hypoxic conditions were achieved at approximately 30 h, as indicated by methylene blue decolorization. OD₆₀₀ (open squares), succinate concentration (solid diamonds), β-galactosidase (β-gal) activity (in Miller units [MU]; open triangles) are shown. The experiments were carried out in biological triplicate, and the results shown are mean values and standard deviations.