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ExPEC O78-9 is serum resistant. Cultures of E. coli strains O78-9 (A) and K-12 (B) were grown overnight and diluted in MOPS medium or in MOPS medium containing 40% serum, and growth curves were generated by measuring OD600 with the BIO-TEK Eon platform. Viability was estimated by dropping 10 µl of a culture treated with serum for 60 min on LB medium plates and incubating them overnight at 37°C (insets). S, serum; IS, inactive serum.
