Table 1.
Mitochondrial and peroxisomal β-oxidation genes in this study.
| Gene names | NCBI accession number | Primers (5′→3′) | Sizes (bp) |
|---|---|---|---|
| Ech1 | NM_016772.1 | Forward: GGAGGGAGTTGGTGGAAT | 291 |
| Reverse: CACAGGCAGAAACGAGGT | |||
| Acox1 | NM_015729 | Forward: CCGCCTATGCCTTCCACT | 182 |
| Reverse: ACCGCAAGCCATCCGACA | |||
| Ehhadh | NM_023737.3 | Forward: TGGACCATACGGTTAGAG | 213 |
| Reverse: CAATCCGATAGTGACAGC | |||
| Acaa1 | NM_130864.3 | Forward: GATGACCTCGGAGAATGTGG | 188 |
| Reverse: CCTGAGACACGGTGATGGT | |||
| PPARα | NM_011144 | Forward: TACTGCCGTTTTCACAAGTGC | 122 |
| Reverse: AGGTCGTGTTCACAGGTAAGA | |||
| PGC1α | NM_008904.2 | Forward: ACAGCAAAAGCCACAAAG | 259 |
| Reverse: TAAGGTTCGCTCAATAGTC | |||
| CPT-1A | NM_013495.2 | Forward: CGTGACGTTGGACGAATC | 165 |
| Reverse: TCTGCGTTTATGCCTATC | |||
| CPT-1B | NM_009948.2 | Forward: GCACACCAGGCAGTAGCTTT | 107 |
| Reverse: CAGGAGTTGATTCCAGACAGGTA | |||
| UCP-1 | NM_009463 | Forward: AGGCTTCCAGTACCATTAGGT | 133 |
| Reverse: CTGAGTGAGGCAAAGCTGATTT | |||
| AMPK | NM_001013367 | Forward: GTCAAAGCCGACCCAATGATA | 100 |
| Reverse: CGTACACGCAAATAATAGGGGTT | |||
| β-Actin | NM_007393 | Forward: GTGACGTTGACATCCGTAAAGA | 245 |
| Reverse: GCCGGACTCATCGTACTCC |