Figure 5. Stu2p function tolerates variation in the primary sequence and in the length of the TOG1-TOG2 linker.
Rescue assays were performed as in Figure 4, using dimerization-impaired rescue constructs. (A) Stu2p variants with ‘shuffled’ (randomized) linker sequences rescue the depletion of endogenous Stu2p comparably to those with the natural linker. (B) Stu2p function is substantially abolished when the TOG1-TOG2 linker is truncated by 60 amino acids. Smaller truncations only show slightly compromised rescue activity. (C) Histogram illustrating the distribution of TOG1-TOG2 linker lengths in ∼300 orthologs. The distribution shows that the linker length can vary but has a minimum tolerable length of ∼40 amino acids.