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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochem J. 2014 Sep 1;462(2):359–371. doi: 10.1042/BJ20140291

Figure 2. Mild reduction of membrane potential in Surf1−/− heart but not skeletal muscle mitochondria and there is no effect on ATP production in either tissue.

Figure 2

A. ATP production in isolated heart mitochondria using either complex I-linked (pyruvate + malate) or complex II-linked (succinate +rotenone) substrates. B. ATP production in isolated skeletal muscle mitochondria using either complex I-linked (glutamate + malate) or complex II-linked (succinate +rotenone) substrates. C. Membrane potential was measured by the change in Safranin O fluorescence using either complex I-linked substrates (pyruvate +malate) or complex II-linked substrates (succinate + rotenone) in isolated heart mitochondria. Data are shown as fluorescent units ×1000. D. Membrane potential was measured by the change in Safranin O fluorescence using either complex I-linked substrates (glutamate +malate) or complex II-linked substrates (succinate + rotenone) in isolated skeletal muscle mitochondria. N=5–10 per experimental group, data are means ± SEM and significance determined by 2-tailed t-test, *p<0.05. pyr=pyruvate, mal=malate, suc=succinate, rot=rotenone