Table 1. Isolated mitochondrial respiration in heart and skeletal muscle from Surf1−/− and wild-type mice.
State 3, state 4, respiratory control ratio (RCR) and ADP to oxygen ratio (P:O) were measured in isolated heart or hind-limb skeletal muscle mitochondria. N=6–10 per experimental group, data are means ± SEM and significance determined by 2-tailed t-test.
| Tissue | Genotype | State 3 | State 4 | RCR (State3/State4) |
ADP:O |
|---|---|---|---|---|---|
| Heart | Wild-type | 325±27 | 58±5 | 5.3±0.5 | 1.98±0.2 |
| Surf1−/− | 264±17 * | 59±6 | 4.8±0.6 | 2.10±0.2 | |
| Skeletal | Wild-type | 548±58 | 53±3 | 10.5±1.3 | 3.38±0.4 |
| Muscle | Surf1−/− | 527±44 | 52±3 | 10.7±1.6 | 2.93±0.4 |