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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Int J Obes (Lond). 2013 Jun 7;38(2):315–320. doi: 10.1038/ijo.2013.96

Figure 1.

Figure 1

Lipid accumulation in mature adipocytes with respect to media height. (A) Lipid content in three pre-adipocyte cell lines after differentiation in 24-well plates using three media volumes. (B) 3T3-L1 cells differentiated in a tilting flask accumulate lipids differently at different depths. (C) Representative pictures of mature 3T3-L1 adipocytes differentiated using three volumes. Cells stained with oil red O (lipid droplet) and hematoxylin (nucleus), shown at 200X magnification.