Figure 1.
Lipid accumulation in mature adipocytes with respect to media height. (A) Lipid content in three pre-adipocyte cell lines after differentiation in 24-well plates using three media volumes. (B) 3T3-L1 cells differentiated in a tilting flask accumulate lipids differently at different depths. (C) Representative pictures of mature 3T3-L1 adipocytes differentiated using three volumes. Cells stained with oil red O (lipid droplet) and hematoxylin (nucleus), shown at 200X magnification.