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Expression of adipocyte-specific markers in relation to media heights during differentiation. (A – C) Gene expression of adipocyte-specific markers over time during 3T3-L1 differentiation process. (D) Leptin secretion over 48 hours from 3T3-L1 cells differentiated in different volumes. (E) Adiponectin secretion from both 3T3-L1 and OP9 adipocytes differentiated in different volumes (1500, 650, and 325μL). *p<0.05; **p<0.01; †p<0.001
