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. 2013 Apr 15;8(11):1016–1024. doi: 10.3969/j.issn.1673-5374.2013.11.007

Figure 1.

Figure 1

Effect of exogenous Activin A on the survival percentage of PC12 cells subjected to oxygen-glucose deprivation (OGD) for 6 hours.

The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay was used to assess cell viability. PC12 cells at a density of 5 × 104 cells/well were treated with 10, 20, 30, 50 and 100 ng/mL exogenous Activin A in a 96-well plate for 24 hours. The survival percentage of PC12 cells in the exogenous Activin A plus OGD group was much higher than that in the OGD and control groups. Cell viability percentage = (absorbance of experimental group/ absorbance of control group) × 100%. Data are expressed as mean ± SD of three independent experiments. One-way analysis of variance was used to compare the mean values. aP < 0.05, vs. control group; bP < 0.05, vs. OGD 6 h and 50 ng Activin A + OGD 6 h groups.

I: Control; II: OGD 6 h; III: Activin A 10 ng + OGD 6 h; IV: Activin A 20 ng + OGD 6 h; V: Activin A 30 ng + OGD 6 h; VI: Activin A 50 ng + OGD 6 h; VII: Activin A 100 ng+ OGD 6 h; h: hours.