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Identification and expression of plasmid pEGFP-N1-MeCP2.
(A) The pEGFP-N1-MeCP2 plasmid was identified by digestion with Pst I and Xho I. M: Marker.
(B) The EGFP-MeCP2 fusion protein was detected by western blot using anti-EGFP antibody. 1: EGFP-SH-SY5Y cells (SH-SY5Y cells transfected with pEGFP-N1); 2: O-MeCP2-SH-SY5Y cells (SH-SY5Y cells transfected with pEGFP-N1-MeCP2). MeCP2: X-linked methyl-CpG binding protein 2.
