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. 2013 Jul 25;8(21):1948–1957. doi: 10.3969/j.issn.1673-5374.2013.21.003

Figure 5.

Figure 5

Effect of X-linked methyl-CpG binding protein 2 (MeCP2) on the viability and apoptosis of 6-hydroxydopamine-treated SH-SY5Y cells.

Control group (Ctrl): Untreated cells; model group: cells incubated with 6-hydroxydopamine (50 μmol/L) for 24 hours; MeCP2 group: cells transfected with pEGFP-N1-MeCP2 for 24 hours, followed by treatment with 6-hydroxydopamine (50 μmol/L) for 24 hours.

(A) Cell viability was quantified using the Cell Counting Kit-8 assay. Absorbance at 540 nm was monitored and the results were normalized to those of control cells.

(B) Flow cytometry analysis of apoptosis.

(C) Quantitative analysis of cell apoptosis.

Data are expressed as mean ± SD of three independent experiments. aP < 0.05, bP < 0.01, vs. control group; cP < 0.05, dP < 0.01, vs. MeCP2 group using one-way analysis of variance followed by the Student Newman-Keuls post hoc test.