Figure 3.

Effects of paeonol on nitrite production in lipopolysaccharide (LPS)-stimulated microglial cells.

Primary microglial cells were incubated in the absence (control; Cont) or presence of LPS (100 ng/mL). The cells were pretreated (A, B) with the indicated amounts of paeonol and LPS was added 30 minutes later. After 24 hours, the cultures were subjected to a nitrite assay (A) and a cell viability assay (B). As a reference, cells were treated with LPS or paeonol only for 24 hours, and the cultures were subjected to nitrite quantification (C). Data are expressed as mean ± SEM from triplicate assays. ^aP < 0.001, vs. the LPS-only treated group (Student's paired t-test). Microglial cells incubated in the absence of LPS for 24 hours served as Cont. M: mol/L.