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. 2013 Apr 5;8(10):869–881. doi: 10.3969/j.issn.1673-5374.2013.10.001

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Morphology of neuron-specific enolase-positive cells (arrows) after induction of bone marrow mesenchymal stem cells (immunohistochemical staining, × 200, streptavidin biotin-peroxidase complex method).

In the epidermal growth factor + basic fibroblast growth factor group (A) and epidermal growth factor + basic fibroblast growth factor + thrombospondin 1 group (B), neuron-specific enolase-positive nuclei were stained dark blue, and the cytoplasm was stained as brown-yellow. The control group (C) showed no pigmentation.