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. 2013 Dec 15;8(35):3306–3315. doi: 10.3969/j.issn.1673-5374.2013.35.005

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bushen Yisui Capsule reduced microtubule-associated protein 2 (MAP-2) expression in the brain of experimental autoimmune encephalomyelitis mice (immunofluorescence staining, fluorescence microscope).

At day 20 after immunization, MAP-2 expression in the model group (B) of mice was significantly decreased compared with the normal group (A), while the expression level in the Bushen Yisui Capsule medium-dose (D) and hormone (C) groups was significantly increased compared with the model group. Change in expression level at day 40 was similar to that at day 20. Fluorescent staining was fluorescein isothiocyanate (FITC) (green) and cell nuclei were stained blue by 4′,6-diamidino-2-phenylindole (DAPI). Scale bars: 100 μm.