Electrophoresis of PCR fragments of the interleukin-18 polymorphisms (–607C/A and –137G/C) in ischemic cerebrovascular disease patients.
(A) The –607C/A polymorphism. M: Marker. Lanes 1, 3, and 5: three samples analyzed with specific primer 1 (matching allele A), intra-control forward primer, and general reverse primer. Lanes 2, 4, and 6: three samples analyzed with specific primer 2 (matching allele C), intra-control forward primer, and general reverse primer. The genotype of the three samples is CA, AA, and CC. The length of specific and reference products are 196 bp and 301 bp, respectively.
(B) The –137G/C polymorphism. M: Marker. Lanes 1, 3, and 5: three samples analyzed with specific primer 1 (matching allele G), intra-control forward primer, and general reverse primer. Lanes 2, 4, and 6: three samples analyzed with specific primer 2 (matching allele C), intra-control forward primer, and general reverse primer. The genotype of the three samples is GC, GG, and CC. The length of specific and reference products are 261 bp and 446 bp, respectively.