Figure 2.

Calcium-induced activation of proteasome in the sciatic nerves following nerve injury.

(A) Following axotomy, the distal stump of the sciatic nerves was processed to measure calpain and proteasome activity. Each time point indicates the time after axotomy (n = 3; mean ± SD). aP < 0.05, vs. uncut control nerves (0 hour, 100 %). (B) Levels of calpain and proteasome activity in sciatic nerve explant cultures. Sciatic nerve explants were cultured for 2 days in the presence or absence of ethylene glycol tetraacetic acid (EGTA; 5 mmol/L) and MG132 (20 μmol/L), and the activities were measured. At least three independent experiments were performed for each condition. -: No treatment. aP < 0.05, vs. uncut control nerves. Differences in the means between groups were statistically assessed using one-way analysis of variance followed by Bonferroni post hoc test.