Figure 5.

Effect of environmental enrichment exposure on c-Fos/doublecortin double-positive cells in layer II of the medial prefrontal cortex of guinea pigs (immunofluorescence staining).

Images immunostained with doublecortin (green), c-Fos (red) and DAPI (blue) showing expression of c-Fos/doublecortin double-positive cells in the control group (A–C) at 0 day (D–F), 10 days (G–I) and 20 days (J–L) post-environmental enrichment. (C, F, I, L) The merged high mag-nification images of frames A′–B′, D′–E′, G′–H′ and J′–K′ in A–B, D–E, G–H and J–K, respectively. The arrowheads indicate c-Fos/doublecortin double labeled cells; the arrows indicate doublecortin-positive cells without c-Fos colocalization. The magnification of the objective lens was 10 × in A,B, D, E, G, H, J, K and 20 × in C, F, I, L.

Samples were visualized via fluorescent microscopy (Nikon, Eclipse 80i). Scale bar = 50 μm in C, F, I, L; 100 μm in A, B, D, E, G, H, J, K.