Figure 1.

Effects of Wen Dan Tang on Ghrelin expression in the prefrontal cortex and hypothalamus (immunohistochemistry staining, × 400).

In the prefrontal cortex and hypothalamus, positive reactions for Ghrelin were focused inside the cells. Immunoreactive products were brown, and small brown granules can be seen in the cytoplasm of positive cells. No specific color changes were visible in the negative control. In the control group, both the prefrontal cortex and hypothalamus displayed a large amount of positive cells, with deeper and more even staining in the cytoplasm. Cell shape was normal. In the model group, the amount of positive cells in the prefrontal cortex and hypothalamus was less, staining was light, and the cytoplasm was not evenly stained. Cell shape was disrupted, with rupturing in some of the cells, especially in the prefrontal cortex. In the diazepam and Wen Dan Tang groups, large amounts of positive cells were observed in both the prefrontal cortex and hypothalamus. Staining was deep and uniform within the cytoplasm. Cell shape was slightly disordered, and a few cells were ruptured.