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. 2013 Dec 5;8(34):3173–3182. doi: 10.3969/j.issn.1673-5374.2013.34.001

Figure 2.

Figure 2

Time course of protein levels of phosphorylated glycogen synthase kinase 3 (p-GSK-3β), GSK-3β, phosphorylated protein phosphatase 2A (p-PP2A) and PP2A in rat hippocampus, and the interaction between tau, GSK-3β and PP2A during brain ischemia and reperfusion.

(A, C) Western blot assay of p-GSK-3β and total protein levels of GSK-3β in brain ischemia (A) and during reperfusion (C). (E, G) Western blot assay of the protein levels of p-PP2A and PP2A in brain ischemia (E) and during reperfusion (G). (I, K) Interaction between tau, GSK-3β and PP2A in brain ischemia (I) and during reperfusion (K).

(B, D, F, H, J, L) Semiquantitative representation of protein levels of p-GSK-3β, GSK-3β, p-PP2A and PP2A and the interaction between tau, GSK-3β and PP2A in A, C, E, G, I and K. Data are expressed as mean ± SD at each time point. aP < 0.05, vs. sham-operated group; bP < 0.05, vs. 15-minute ischemia group, n = 8. I: Ischemia; R: reperfusion; IP: immunoprecipitation; WB: western blot; min: minute; h: hour.