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. 2014 May 1;9(9):959–967. doi: 10.4103/1673-5374.133140

Figure 2.

Figure 2

Effects of lipopolysaccharide on IL-1β and TNF-α secretion by microglia (enzyme linked immunosorbent assay).

(A) IL-1β levels in medium after incubation with 100 ng/mL lipopolysaccharide. (B) TNF-α levels in medium after incubation with 100 ng/mL lipopolysaccharide. Data are expressed as mean ± SD. The experiment was performed in triplicate. Completely random design was employed in analysis of variance. Paired comparison was done using least significant difference test. *P < 0.05, **P < 0.01, vs. control group; #P < 0.05, vs. the previous time point. 1, 3, 6, 12, 24 h: 1, 3, 6, 12, 24 hours after incubation with lipopolysaccharide. IL-1β: Interleukin-1β; TNF-α: tumor necrosis factor-α.