Figure 2.

Western blot analysis of P2X₇ receptor (P2X₇-R) protein levels in whole retina after intraocular pressure (IOP) elevation.

(A) Representative immunoreactive bands of intracellular and extracellular P2X₇ receptors in normal, sham control, and treated retinas on days 1, 2, and 3 after IOP elevation. (B) Densitometric quantification of immunoreactive bands of intracellular (left) and extracellular (right) P2X₇ receptor in sham control and treated retinas on days 1, 2, and 3 compared to normal retina. Data are expressed as mean ± SD (n = 3). The asterisks indicate significant differences from the sham control eyes (unpaired t-test, *P < 0.05, **P < 0.01). From Sugiyama et al. (2013).