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Three-dimensional collagen gel culture of retinal explants.
Retinas were isolated under sterile conditions, and cut into square pieces with a sharp razor blade. The retinal explants embedded into type I liquid collagen solution on ice. After warming at 37°C, the retinal explants were fixed in collagen gels. The explants were cultured in se-rum-free media at 37°C and exposed to 5% CO2.
