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. 2014 Apr 1;9(7):727–734. doi: 10.4103/1673-5374.131577

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Presence of Ku70 and TUNEL/Bax in the cortex of rats (immunofluorescence double staining).

(A) Presence of Ku70 and TUNEL in the cortex of rats of the electric stimulation group. Ku70-positive cells (red, A1), TUNEL (green, A2) and 4’,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue, A3), and all three merged (A4). Ku70 did not co-localize with TUNEL-positive cells. (B) Presence of Ku70 and Bax in the cortex 72 hours after cerebral ischemia/reperfusion injury in the model group. Immunofluorescence staining for Bax (red, B1) and Ku70 (green, B2), DAPI nuclear staining (blue, B3), and all three merged (B4). Ku70 co-localized with Bax in the cytoplasm. (C) Presence of Ku70 and Bax in the cortex 72 hours after cerebral ischemia/reperfusion injury in the electric-stimulating group. Immunofluorescence staining for Bax (red, C1) and Ku70 (green, C2), DAPI nuclear staining (blue, C3), and all three merged (C4). Ku70 co-localized with Bax in the cytoplasm. Fastigial nucleus-stimulation was given to rats 72 hours before cerebral ischemia/reperfusion injury. Scale bars: 20 μm.