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. 2014 Jun 15;9(12):1241–1248. doi: 10.4103/1673-5374.135333

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neural differentiation of BMSCs on the 6^th day of in vitro transfection.

(A, B) Immunofluorescence for the neuronal markers β-III tubulin (A1 and A3; red) and MAP-2 (B1 and B3; green) in BMSCs transfected with pLVX-EN-rno-miR124 (miR-124⁺) after 6 days of in vitro differentiation. Scale bars: 100 μm. BMSCs developed dendrites and neurites, similar to neurons (A2, A4, B2, B4). Scale bars: 100 μm. (C) Western blotting was used to detect the expression of synaptophysin (38 kDa). BMSCs: Bone marrow-derived mesenchymal stem cells; MAP-2: microtubule-associated protein-2.