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. 2014 May 15;9(10):1047–1054. doi: 10.4103/1673-5374.133169

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The structure of the blood-retinal barrier of adult mice under light microscopy by gelatin-ink (black) perfusion, collagen IV and glial fibrillary acidic protein secondary antibody Alex Fluor 568 (red) immunofluorescence staining.

(A) The blood vessel lumen and endothelium is shown by gelatin-ink perfusion under a light microscope. (B) The basal lamina of the vessel is shown by anti-collagen (Col) IV immunofluorescence staining under a light microscope. (C) The astrocytic processes enveloping the retinal vessels are shown by anti-glial fibrillary acidic protein (GFAP) immunofluorescence staining under a light microscope. Scale bars: 100 μm.