Figure 3.

Level of agreement between in vivo (NIRS) and in situ (high-resolution respirometry) indices of skeletal muscle mitochondrial respiratory capacity

Bland–Altman plots (difference vs. average) of in vivo and in situ measurements of skeletal muscle mitochondrial respiratory capacity from the vastus lateralis muscle in healthy adults. Because the two measurement methods have differing units of value, we calculated standardized Z-scores for the study sample. Complex I+II (A, glutamate+malate+succinate; D, palmitoyl-l-carnitine+malate+glutamate+succinate), Complex I only (B, glutamate+malate; E, palmitoyl-l-carnitine+malate+glutamate) and Complex II only (C, glutamate+malate+succinate+rotenone; F, palmitoyl-l-carnitine+malate+glutamate+succinate+rotenone); n = 21 for A–C; n = 17 for D–F. Dashed lines represent the 95% limits of agreement (mean ± 1.96SD), for which 95% of measurements are expected to lie between.