(a–b) Representative FACS plots showing frequency of c-kit+Sca-1+ mononuclear cells in Lineage-bone marrow (LSK) from Fbxw7F/+Mx1Cre+, Fbxw7mut/+Mx1Cre+, or Fbxw7F/FMx1Cre+ mice harvested 8 weeks after pI:pC injection, and number of phenotypic LT-HSC (CD150+CD48−LSK) recovered (b). (c) Primary and secondary colonies derived from LSK cells sorted from bone marrow of the indicated genotype. Mean +/− SD of 3 replicates shown. *p <0.05 **p<0.01. (d) Frequency of CD45.2+ donor-derived peripheral blood mononuclear cells (PBMC) in lethally-irradiated recipient mice transplanted with 5×105 total BMMC from either Fbxw7F/+Mx1Cre+, Fbxw7mut/+Mx1Cre+, or Fbxw7F/FMx1Cre+ mice (CD45.2+) mixed at 1:1 ratio with wild-type BMMC (CD45.1+), at 4 and 8 weeks post-transplant. (d) FACS profiling of thymocytes from Fbxw7 wild type, mutant or knock-out mice. Mice were analyzed four weeks after pI:pC injections. (e–f) Histogram and calculated mean fluorescence intensity (MFI) (f) of GFP measured by FACS in LT-HSC from mice in D–F expressing a c-Myc:GFP fusion (MycGFP/+) allele.