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. 2014 Aug 27;9(8):e104334. doi: 10.1371/journal.pone.0104334

Figure 3. Detection of target DNA within various concentrations of gDNA by MAMEF.

Figure 3

Various concentrations of C. difficile gDNA (strain CD630; ATCC, USA) were tested in the MAMEF platform. Concentrations of 10 ng and 5 ng were microwave irradiated for 8 seconds at 70% power. gDNA was subjected to disruption to increase the chances of the detection probes accessing the toxin specific sequences. The data presented is the result from a single reproduced assay. (A) The fluorescent signal intensities generated from toxin A detection within microwaved gDNA (excitation at 495 nm, emission at 519 nm). (B) The fluorescent signal intensities generated from toxin B detection within gDNA (excitation at 590 nm, emission at 617 nm). The control was PBS buffer.