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. 2014 Aug 27;9(8):e104334. doi: 10.1371/journal.pone.0104334

Figure 5. Detection of DNA from microwaved C. difficile spore preparation in PBS using MAMEF.

Figure 5

Spores of C. difficile from strain CD630 at a range of concentrations 10, 100, 1000 and 10 000 cfu were suspended in PBS and subjected to microwave irradiation and screened for the presence of toxin A and toxin B using MAMEF. (A) Various fluorescent signal intensities generated from detection of toxin A within gDNA from irradiated spores (excitation at 495 nm, emission at 519 nm). (B) The various fluorescent signal intensities generated from detection of toxin B within gDNA from irradiated spores (excitation at 590 nm, emission at 617 nm).