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. 2014 Aug 27;9(8):e104334. doi: 10.1371/journal.pone.0104334

Figure 6. Detection of DNA from lysed C. difficile in feces using MAMEF.

Figure 6

Spores of C. difficile from strain CD630 at a range of concentrations 10, 100, and 1000 cfu were suspended in human feces and subjected to microwave irradiation, then screened for the presence of toxin A and toxin B using MAMEF. (A) The various fluorescent signal intensities generated from detection of toxin A within gDNA from irradiated spores of C. difficile in human feces (excitation at 495 nm, emission at 519 nm). (B) Graph demonstrating the fluorescent signal intensities generated from detection of toxin B within gDNA from irradiated spores of C. difficile in human feces (excitation at 590 nm, emission at 617 nm).