Table 1. Table of Oligonucleotides.
Toxin A | Toxin B | |
Anchor Probe | Thiol-TTTTT-TTTAATACTAACACTGC | Thiol-TTTTTT-CAAGACTCTATTATAG |
Capture Probe | Alexa-488-TGTTGCAGTTACTGGATGGCAA | Alexa-594-TAAGTGCAAATCAATATGAAG |
Synthetic oligonucleotides | AAATTATGATTGTGACGTAATCCCAATACAACGTCAATGACCTACCGTT | AGTTCTGAGATAATATCTAATCCCAATATTCACGTTTAGTTATACTTG |
Anchor and capture probes, and synthetic oligonucleotides target regions used in the MAMEF assay for toxins A and B are shown, along with the corresponding fluorophore used.