Dopamine (DA) is first deposited onto pristine Ti to form DA-Ti substrate. Then a suspension of anionic nanoparticles, formed through the electrostatic interaction between poly-lysine (PLL) and heparin (Hep), is deposited onto DA-Ti to form PLL/Hep-Ti, through the Schiff base conjugation reaction between PLL and DA as well as the electrostatic interaction between Hep and DA. Finally, cationic chitosan (CH) and methotrexate (MTX) are simultaneously immobilized onto the anionic PLL/Hep-Ti to form CH-MTX-Ti substrates through electrostatic interaction. The CH- MTX-Ti substrates are interacted with GTCs and bacteria to confirm their anti-cancer and anti-bacteria capability, respectively. Abbreviation in the schematic illustration: Ti=Titanium, DA=Dopamine, PLL/Hep=Poly-lysine/Heparin, CH=Chitosan, MTX=Methotrexate