FIG. 4.

SNAI1 expression and CTNNB1 localization at different culture stages. Immunofluorescence staining of EDCs, CSps, and EDCs plated to form CSps in the presence of TGFβ (5 nM) or SB431542 (10 μM), and CDCs for SNAI1 (SNAIL, green), CTNNB1 (βcatenin, red), and Hoechst (blue). Confocal images were acquired with a 40×oil immersion objective, with the same settings and from the same set of stainings. For CSps, 1 representative slice has been selected out of a stack of 10–15 slices (1.5 μm step). Color images available online at www.liebertpub.com/scd