Figure 2. Binding of phage clones to mAb K20.

Plates were sensitized with a mAb directed against phage protein III (1 µg/ml) and 100 µl of purified phage clones (10¹¹ pfu/ml) were added. After 1 h incubation, mAb K20 or an isotype-matched IgM (1 µg/ml) were added. Binding was detected by using alkaline phosphatase-conjugated anti-mouse IgM Ab. Pools of libraries, (pVIII-12aa/pVIII.Cys-Cys or pVIII-9aa.Cys/pVIII.Cys-Cys or pVIII-9aa/pVIII-9aa.Cys) were used in different phage selections. Results are reported in separate panels (A, B or C). Phage pC89, displaying wild-Type pVIII, was used as negative control. Data represents the means ± the SD of three determinations.