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. 2014 Aug 27;9(8):e105697. doi: 10.1371/journal.pone.0105697

Figure 1. Suppressive effects of TIMP-2 on the changes in TF and TM expression and MT1-MMP activity in response to TNF-α.

Figure 1

(A and B) TNF-α-induced TF and TM expression in a dose dependent manner. ECs were treated with TNF-α (1 to 50 ng/mL) for 18 hours. Immunoblots are from an experiment representative of 3 similar experiments. (C) Effect of TNF-α on MT1-MMP activity. ECs were pretreated with 40 nmol/L TIMP-2 for 60 min and then stimulated by TNF-α (10 ng/mL), and thereafter MT1-MMP activity was determined as described in the Methods section. Results are expressed as means±SD of 4 separate experiments. * P<0.05, ** P<0.005. The lower panel showed the protein levels of MT1-MMP as determined by Western blotting. (D–F) Effects of TIMP-2 on TNF-α-induced changes in TF and TM expression. ECs were pretreated with TIMP-2 for 60 min and then stimulated by TNF-α, followed by Western blotting and TF activity assay. Results are from an experiment representative of 3 similar experiments. Quantitative results of TF and TM were obtained by densitometry. * P<0.05, ** P<0.005.