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. 2014 Aug 27;9(8):e105797. doi: 10.1371/journal.pone.0105797

Figure 2. Representative 2DE gel of soluble proteins in A549 cells stained with CBB.

Figure 2

Each protein sample (1 mg) was separated by IEF in a 24 cm long IPG gel strip containing a wide-range linear pH gradient of 3–10, followed by SDS-PAGE on a vertical 12% gel. Proteins differentially expressed in response to excess Zn (100 µM ZnSO4 for 24 h) are illustrated with a diamond symbol. These proteins showed a two-fold or greater difference in abundance (p≤0.05) in A549 cells. Other proteins identified are labeled with a ring symbol.