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. 2014 Aug 27;9(8):e105797. doi: 10.1371/journal.pone.0105797

Figure 5. Time-dependent changes in ZIP-1, ZnT-1, MT-1 and MTF-1 transcript abundance in cells treated with ZnSO4.

Figure 5

(A) RT-PCR analysis of ZIP-1, ZnT-1, MT-1 and MTF-1 mRNA in A549 cells after treatment for 0, 6, 8, 10, 12, 24 and 48 h with exogenous 100 µM ZnSO4; (B) Band density was analyzed with Gel Image Analysis software (CLINX, Shanghai, China). GAPDH expression was used as the internal control. Data was normalized and mean values ± SD were calculated from at least three independent samples.