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. 2014 Aug 27;9(8):e106032. doi: 10.1371/journal.pone.0106032

Figure 7. RBP2 activates HIF-1α via the PI3K/Akt signaling pathway.

Figure 7

(A) Silencing RBP2 expression in H1975 cells significantly decreased the phosphorylation of Akt, and the forced expression of RBP2 in SK-MES-1 cells increased the activity of Akt. (B) When Akt was constitutively activated in RBP2-siRNA2 H1975, the expression of HIF-1α and VEGF were increased compared to the control. The PI3K/Akt inhibitor LY294002 significantly inhibited the expression of HIF-1α and VEGF in pcDNA3-HA-RBP2 SK-MES-1 cells. (C) Westernblots showing the time course of Akt phosphorylation in RBP2-siRNA2 H1975 cells due to VEGF-165 (25 ng/mL). (D) In the presence of recombinant human VEGF-165 stimulation, the activation of Akt was increased in RBP2-siRNA2 H1975 cells and RBP2-overexpressing SK-MES-1 cells (25 ng/mL, 30 minutes).