Figure 3. Knockdown of Laf4 causes a defect in the migration of cortical cells.

E14 mouse brains were electroporated with shRNA-Laf4 or shRNA-scramble vectors with a control (IRES-GFP) or the Laf4-rescue-IRES-GFP vector and cultured for six days before re-sectioning for image analysis (A). The average percentage of GFP⁺ cells in 5 zones (example zones Z1–Z5 shown) spanning from the ventricle (V) to the pial border was quantified (B). Brains electroporated with shRNA-Laf4 construct show a significant reduction in GFP⁺ cells corresponding to migrating cells to the superficial layers of the cortex; this phenotype is almost completely reversed by co-electroporation with a Laf4-rescue-IRES-GFP construct. (n = 6 brains quantified for each vector combination; *p<0.05, **p<0.01 (2-way ANOVA Bonferroni’s multiple comparison test). Scale bar = 50 µM.