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. 2014 Aug 27;9(8):e105933. doi: 10.1371/journal.pone.0105933

Figure 6. Over-expression of Mdga2 rescues the deficit of cortical cells migration generated by Laf4 knockdown.

Figure 6

(A) E14 mouse brains were electroporated with shRNA-Laf4 or shRNA-scramble vectors with the control IRES-GFP or an Mdga2-IRES-GFP vector and cultured for six days before re-sectioning for image analysis. The average percentage of GFP+ cells in 5 zones spanning from the ventricle (V) to the pial border was quantified (B). Co-electroporation of shRNA-Laf4 and Mdga2-IRES-GFP constructs results in rescue of the migration defect observed using shRNA-Laf4 alone. (n = 4 brains quantified for each vector combination; *p<0.05, **p<0.01, ***p<0.001, 2-way ANOVA Bonferroni’s multiple comparison test).